An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Interaction of the transforming acidic coiled-coil 1 (TACC1) protein with ch-TOG and GAS41/NuBI1 suggests multiple TACC1-containing protein complexes in human cells.

Lauffart B, Howell SJ, Tasch JE, Cowell JK, Still IH

Dysregulation of the human transforming acidic coiled-coil (TACC) proteins is thought to be important in the evolution of breast cancer and multiple myeloma. However, the exact role of these proteins in the oncogenic process is currently unknown. Using the full-length TACC1 protein as bait to screen a human mammary epithelial cDNA library, we have identified two genes that are also ... [more]

Biochem. J. Apr. 01, 2002; 363(0);195-200 [Pubmed: 11903063]

Throughput

Low Throughput

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
TACC1 YEATS4	Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.	Lauffart B (2002)	Low	-	BioGRID	-

Curated By

BioGRID

Interaction Summary

YEATS4

Gene Ontology Biological Process

Gene Ontology Molecular Function

DNA binding [TAS]protein C-terminus binding [IPI]protein binding [IPI]sequence-specific DNA binding transcription factor activity [TAS]structural constituent of cytoskeleton [NAS]

Gene Ontology Cellular Component

TACC1