BAIT
RFFL
CARP-2, CARP2, FRING, RIFIFYLIN, RNF189, RNF34L
ring finger and FYVE-like domain containing E3 ubiquitin protein ligase
GO Process (10)
GO Function (6)
GO Component (7)
Gene Ontology Biological Process
- negative regulation of cysteine-type endopeptidase activity involved in execution phase of apoptosis [IMP]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [IMP]
- negative regulation of signal transduction by p53 class mediator [IMP]
- negative regulation of tumor necrosis factor-mediated signaling pathway [IMP]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IMP]
- protein K48-linked ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IMP]
- regulation of TOR signaling [IC]
- regulation of fibroblast migration [ISS]
- ubiquitin-dependent protein catabolic process [IDA, IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Homo sapiens
PREY
RAB13
GIG4
RAB13, member RAS oncogene family
GO Process (15)
GO Function (4)
GO Component (14)
Gene Ontology Biological Process
- GTP catabolic process [IBA]
- Rab protein signal transduction [IBA]
- cellular response to insulin stimulus [ISS]
- cortical actin cytoskeleton organization [IBA, ISS]
- endocytic recycling [IMP]
- endosomal transport [IMP]
- endothelial cell chemotaxis [ISS]
- establishment of Sertoli cell barrier [ISS]
- establishment of protein localization to plasma membrane [IMP]
- intracellular protein transport [IBA]
- membrane organization [TAS]
- neuron projection development [IMP]
- protein kinase A signaling [IMP]
- tight junction assembly [IMP]
- trans-Golgi network to recycling endosome transport [ISS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- Golgi apparatus [IDA]
- cytoplasm [IDA]
- cytoplasmic vesicle [IDA]
- cytoplasmic vesicle membrane [TAS]
- endocytic vesicle [IDA]
- extracellular vesicular exosome [IDA]
- insulin-responsive compartment [ISS]
- lamellipodium [ISS]
- lateral plasma membrane [IDA]
- neuron projection [IDA]
- plasma membrane [IDA]
- recycling endosome [IDA]
- tight junction [IDA]
- trans-Golgi network [IDA]
Homo sapiens
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
The integral function of the endocytic recycling compartment is regulated by RFFL-mediated ubiquitylation of Rab11 effectors.
Endocytic trafficking is regulated by ubiquitylation (also known as ubiquitination) of cargoes and endocytic machineries. The role of ubiquitylation in lysosomal delivery has been well documented, but its role in the recycling pathway is largely unknown. Here, we report that the ubiquitin (Ub) ligase RFFL regulates ubiquitylation of endocytic recycling regulators. An RFFL dominant-negative (DN) mutant induced clustering of endocytic ... [more]
J. Cell. Sci. Dec. 07, 2018; 132(3); [Pubmed: 30659120]
Throughput
- High Throughput
Additional Notes
- BioID
Curated By
- BioGRID