Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

RBP1 recruits both histone deacetylase-dependent and -independent repression activities to retinoblastoma family proteins.

Lai A, Lee JM, Yang WM, DeCaprio JA, Kaelin WG, Seto E, Branton PE

Retinoblastoma (RB) tumor suppressor family proteins block cell proliferation in part by repressing certain E2F-specific promoters. Both histone deacetylase (HDAC)-dependent and -independent repression activities are associated with the RB "pocket." The mechanism by which these two repression functions occupy the pocket is unknown. A known RB-binding protein, RBP1, was previously found by our group to be an active corepressor which, ... [more]

Mol. Cell. Biol. Oct. 01, 1999; 19(10);6632-41 [Pubmed: 10490602]

Throughput

  • Low Throughput

Curated By

  • BioGRID