BAIT

KCNH5

EAG2, H-EAG2, Kv10.2, hEAG2
potassium voltage-gated channel, subfamily H (eag-related), member 5
Homo sapiens
PREY

MBP

myelin basic protein
GO Process (5)
GO Function (1)
GO Component (0)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Homo sapiens

Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Tubulin as a binding partner of the heag2 voltage-gated potassium channel.

Bracey K, Ju M, Tian C, Stevens L, Wray D

The aim of this work was to investigate interactions of the human ether-a-go-go channel heag2 with human brain proteins. For this, we used heag2-GST fusion proteins in pull-down assays with brain proteins and mass spectrometry, as well as coimmunoprecipitation. We identified tubulin and heat shock 70 proteins as binding to intracellular C-terminal regions of the channel. To study functional effects, ... [more]

J. Membr. Biol. Apr. 01, 2008; 222(3);115-25 [Pubmed: 18458804]

Throughput

  • Low Throughput

Curated By

  • BioGRID