Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A novel PrP partner HS-1 associated protein X-1 (HAX-1) protected the cultured cells against the challenge of H?O?.

Jing YY, Li XL, Shi Q, Wang ZY, Guo Y, Pan MM, Tian C, Zhu SY, Chen C, Gong HS, Han J, Gao C, Dong XP

Prion protein (PrP) is a ubiquitous conserved glycoprotein predominantly expressed in neurons of the central nervous system (CNS). To elucidate on its cellular function, we performed a yeast two-hybrid screen within an adult human brain cDNA library for potential PrP-binding molecules. A novel protein, HS-1 associated protein X-1 (HAX-1), was identified to be able to bind with PrP strongly. The ... [more]

J. Mol. Neurosci. Oct. 01, 2011; 45(2);216-28 [Pubmed: 21301993]

Throughput

  • Low Throughput

Curated By

  • BioGRID