BAIT

PRNP

ASCR, AltPrP, CD230, CJD, GSS, KURU, PRIP, PrP, PrP27-30, PrP33-35C, PrPc, p27-30, RP5-1068H6.2
prion protein
Alzheimer's Disease Project
Homo sapiens
PREY

ATP6V1B2

ATP6B1B2, ATP6B2, HO57, VATB, VPP3, Vma2
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B2
Homo sapiens

Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Publication

A novel PrP partner HS-1 associated protein X-1 (HAX-1) protected the cultured cells against the challenge of H?O?.

Jing YY, Li XL, Shi Q, Wang ZY, Guo Y, Pan MM, Tian C, Zhu SY, Chen C, Gong HS, Han J, Gao C, Dong XP

Prion protein (PrP) is a ubiquitous conserved glycoprotein predominantly expressed in neurons of the central nervous system (CNS). To elucidate on its cellular function, we performed a yeast two-hybrid screen within an adult human brain cDNA library for potential PrP-binding molecules. A novel protein, HS-1 associated protein X-1 (HAX-1), was identified to be able to bind with PrP strongly. The ... [more]

J. Mol. Neurosci. Oct. 01, 2011; 45(2);216-28 [Pubmed: 21301993]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
PRNP ATP6V1B2
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2842670

Curated By

  • BioGRID