An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

SPRED2 deficiency elicits cardiac arrhythmias and premature death via impaired autophagy.

Ullrich M, Assmus B, Augustin AM, Haebich H, Abesser M, Martin Machado J, Werner F, Erkens R, Arias-Loza AP, Umbenhauer S, Wagner H, Benz PM, Unger A, Linke WA, Frantz S, Baba HA, Kuhn M, Schuh K

Cardiac functionality is dependent on a balanced protein turnover. Accordingly, regulated protein decay is critical to maintain cardiac function. Here we demonstrate that deficiency of SPRED2, an intracellular repressor of ERK-MAPK signaling markedly expressed in human heart, resulted in impaired autophagy, heart failure, and shortened lifespan. SPRED2-/- mice showed cardiomyocyte hypertrophy, cardiac fibrosis, impaired electrical excitability, and severe arrhythmias. Mechanistically, ... [more]

J. Mol. Cell. Cardiol. Apr. 01, 2019; 129();13-26 [Pubmed: 30771306]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

SPRED2

Gene Ontology Biological Process

Gene Ontology Molecular Function

protein binding [IPI]protein kinase binding [IPI]protein serine/threonine kinase inhibitor activity [ISS]stem cell factor receptor binding [ISS]

Gene Ontology Cellular Component

CTSD