Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

Publication

A Role for Mitochondrial Translation in Promotion of Viability in K-Ras Mutant Cells.

Martin TD, Cook DR, Choi MY, Li MZ, Haigis KM, Elledge SJ

Activating mutations in the KRAS oncogene are highly prevalent in tumors, especially those of the colon, lung, and pancreas. To better understand the genetic dependencies that K-Ras mutant cells rely upon for their growth, we employed whole-genome CRISPR loss-of-function screens in two isogenic pairs of cell lines. Since loss of essential genes is uniformly toxic in CRISPR-based screens, we also ... [more]

Cell Rep Jul. 11, 2017; 20(2);427-438 [Pubmed: 28700943]

Throughput

  • High Throughput

Additional Notes

  • CRISPR screen analysis showed synthetic lethality with K-Ras mutant in DLD1 cells.
  • Gene loss results in the selective reduction of K-Ras mutant cell growth.
  • Synthetic lethality score > 1.0.
  • Synthetic lethality score > 2.0.

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
KRAS EIF4A3
Negative Genetic
Negative Genetic

Mutations/deletions in separate genes, each of which alone causes a minimal phenotype, but when combined in the same cell results in a more severe fitness defect or lethality under a given condition. This term is reserved for high or low throughput studies with scores.

High-BioGRID
3343154
KRAS EIF4A3
Synthetic Lethality
Synthetic Lethality

A genetic interaction is inferred when mutations or deletions in separate genes, each of which alone causes a minimal phenotype, result in lethality when combined in the same cell under a given condition.

High-BioGRID
1414541

Curated By

  • BioGRID