WDR77
Gene Ontology Biological Process
- negative regulation of cell proliferation [IGI]
- negative regulation of epithelial cell proliferation involved in prostate gland development [IMP]
- positive regulation of cell proliferation [IGI]
- regulation of nucleic acid-templated transcription [ISO]
- regulation of transcription from RNA polymerase II promoter [IMP]
- secretory columnal luminar epithelial cell differentiation involved in prostate glandular acinus development [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
PRMT5
Gene Ontology Biological Process
- circadian regulation of gene expression [IMP]
- endothelial cell activation [ISO]
- histone H4-R3 methylation [IMP]
- histone arginine methylation [IBA]
- negative regulation of cell differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- peptidyl-arginine N-methylation [ISO]
- peptidyl-arginine methylation [ISO]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IBA, ISO]
- positive regulation of oligodendrocyte differentiation [ISO]
- protein methylation [ISO]
- regulation of DNA methylation [ISO]
- regulation of ERK1 and ERK2 cascade [ISO]
- spliceosomal snRNP assembly [ISO]
Gene Ontology Molecular Function- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
MEP50/PRMT5-mediated methylation activates GLI1 in Hedgehog signalling through inhibition of ubiquitination by the ITCH/NUMB complex.
Transcription factor GLI1 is an effecter of Hedgehog (HH) signalling and activated in a broad spectrum of cancers. However, the role of the HH-GLI1 pathway in cancer and the activation mechanism of GLI1 in HH signalling after dissociation from its inhibitor, SUFU, are not fully understood. Here, we found that GLI1 associated with the methylosome protein 50 (MEP50)/protein arginine methyltransferase ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| PRMT5 WDR77 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| PRMT5 WDR77 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | High | 0.941 | BioGRID | 2666258 |
Curated By
- BioGRID