BAIT

NEDD8

NEDD-8
neural precursor cell expressed, developmentally down-regulated 8
Human Papilloma Virus (HPV) Project
UPS Project
Homo sapiens
PREY

CBLB

Cbl-b, RNF56, Nbla00127
Cbl proto-oncogene B, E3 ubiquitin protein ligase
UPS Project
GO Process (2)
GO Function (1)
GO Component (4)

Gene Ontology Biological Process

Gene Ontology Molecular Function

Gene Ontology Cellular Component

Homo sapiens

Protein-peptide

An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.

Publication

Selectivity of the CUBAN domain in the recognition of ubiquitin and NEDD8.

Castagnoli L, Mandaliti W, Nepravishta R, Valentini E, Mattioni A, Procopio R, Iannuccelli M, Polo S, Paci M, Cesareni G, Santonico E

Among the members of the ubiquitin-like (Ubl) protein family, neural precursor cell expressed developmentally down-regulated protein 8 (NEDD8) is the closest in sequence to ubiquitin (57% identity). The two modification mechanisms and their functions, however, are highly distinct and the two Ubls are not interchangeable. A complex network of interactions between modifying enzymes and adaptors, most of which are specific ... [more]

FEBS J. Dec. 01, 2018; 286(4);653-677 [Pubmed: 30659753]

Throughput

  • High Throughput

Additional Notes

  • #LPPI
  • Likely protein-protein interaction
  • Phage display

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
CBLB NEDD8
Reconstituted Complex
Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Low-BioGRID
2621849

Curated By

  • BioGRID