Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

A generic approach for the purification of signaling complexes that specifically interact with the carboxyl-terminal domain of G protein-coupled receptors.

Maurice P, Daulat AM, Broussard C, Mozo J, Clary G, Hotellier F, Chafey P, Guillaume JL, Ferry G, Boutin JA, Delagrange P, Camoin L, Jockers R

G protein-coupled receptors (GPCRs) constitute the largest family of membrane receptors and are major drug targets. Recent progress has shown that GPCRs are part of large protein complexes that regulate their activity. We present here a generic approach for identification of these complexes that is based on the use of receptor subdomains and that overcomes the limitations of currently used ... [more]

Mol. Cell Proteomics Aug. 01, 2008; 7(8);1556-69 [Pubmed: 18448421]

Throughput

  • High Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
MTNR1B TUBB3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

High-BioGRID
-
MTNR1B TUBB3
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

High-BioGRID
-

Curated By

  • BioGRID