ADRB1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
USP20
Gene Ontology Biological Process
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K48-linked deubiquitination [IDA]
- protein K63-linked deubiquitination [IDA]
- protein deubiquitination [IDA]
- regulation of G-protein coupled receptor protein signaling pathway [IMP]
- regulation of proteasomal protein catabolic process [IBA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
The deubiquitinase ubiquitin-specific protease 20 is a positive modulator of myocardial ?1-adrenergic receptor expression and signaling.
Reversible ubiquitination of G protein-coupled receptors regulates their trafficking and signaling; whether deubiquitinases regulate myocardial ?1-adrenergic receptors (?1ARs) is unknown. We report that ubiquitin-specific protease 20 (USP20) deubiquitinates and attenuates lysosomal trafficking of the ?1AR. ?1AR-induced phosphorylation of USP20 Ser-333 by protein kinase A-? (PKA?) was required for optimal USP20-mediated regulation of ?1AR lysosomal trafficking. Both phosphomimetic (S333D) and phosphorylation-impaired ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID