ATXN1
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EHD1
Gene Ontology Biological Process
- cellular response to nerve growth factor stimulus [ISO]
- cholesterol homeostasis [IMP]
- endocytic recycling [ISO]
- endocytosis [IDA, ISO]
- endosomal transport [IDA]
- intracellular protein transport [ISO]
- low-density lipoprotein particle clearance [IMP]
- neuron projection development [ISO]
- positive regulation of cholesterol storage [IMP]
- positive regulation of endocytic recycling [IMP]
- positive regulation of myoblast fusion [IMP]
- protein homooligomerization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- early endosome [IDA]
- early endosome membrane [ISO]
- endocytic vesicle [IDA]
- endosome [IDA]
- endosome membrane [ISO]
- extracellular vesicular exosome [ISO]
- lipid particle [IDA]
- membrane [ISO]
- myelin sheath [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet dense tubular network membrane [IDA]
- recycling endosome membrane [ISO]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATXN1 EHD1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2622434 |
Curated By
- BioGRID