ATXN1
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
HSPA1A
Gene Ontology Biological Process
Gene Ontology Molecular Function- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [ISO]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [ISO]
- protein N-terminus binding [ISO]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [ISO]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [ISO]
- protein N-terminus binding [ISO]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- aggresome [ISO]
- apical plasma membrane [ISO]
- basolateral plasma membrane [ISO]
- blood microparticle [ISO]
- centriole [ISO]
- cytoplasm [IDA, ISO]
- cytosol [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- inclusion body [ISO]
- mitochondrion [ISS]
- nuclear speck [ISO]
- nucleus [ISO]
- perinuclear region of cytoplasm [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
- ubiquitin ligase complex [ISO]
- vesicle [ISO]
Proximity Label-MS
An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATXN1 HSPA1A | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | 2622512 |
Curated By
- BioGRID