Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.

Zhang S, Williamson NA, Bogoyevitch MA

Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]

Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]

Throughput

  • High Throughput

Additional Notes

  • ataxin-1[85Q]

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATXN1 IPO5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

High-BioGRID
2622717

Curated By

  • BioGRID