BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP2A1
SERCA1
ATPase, Ca++ transporting, cardiac muscle, fast twitch 1
GO Process (20)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- apoptotic mitochondrial changes [ISO]
- calcium ion import [ISO]
- calcium ion transmembrane transport [ISO]
- calcium ion transport [ISO]
- carbohydrate derivative transport [ISO]
- intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress [ISO]
- maintenance of mitochondrion location [ISO]
- negative regulation of endoplasmic reticulum calcium ion concentration [ISO]
- negative regulation of striated muscle contraction [ISO]
- nucleotide transmembrane transport [ISO]
- positive regulation of endoplasmic reticulum calcium ion concentration [ISO]
- positive regulation of fast-twitch skeletal muscle fiber contraction [ISO]
- positive regulation of mitochondrial calcium ion concentration [ISO]
- regulation of muscle contraction [TAS]
- regulation of striated muscle contraction [ISO]
- relaxation of skeletal muscle [ISO]
- response to endoplasmic reticulum stress [ISO]
- response to peptide hormone [ISO]
- sarcoplasmic reticulum calcium ion transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID