ATXN1
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EHD1
Gene Ontology Biological Process
- cellular response to nerve growth factor stimulus [ISO]
- cholesterol homeostasis [IMP]
- endocytic recycling [ISO]
- endocytosis [IDA, ISO]
- endosomal transport [IDA]
- intracellular protein transport [ISO]
- low-density lipoprotein particle clearance [IMP]
- neuron projection development [ISO]
- positive regulation of cholesterol storage [IMP]
- positive regulation of endocytic recycling [IMP]
- positive regulation of myoblast fusion [IMP]
- protein homooligomerization [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- early endosome [IDA]
- early endosome membrane [ISO]
- endocytic vesicle [IDA]
- endosome [IDA]
- endosome membrane [ISO]
- extracellular vesicular exosome [ISO]
- lipid particle [IDA]
- membrane [ISO]
- myelin sheath [IDA]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [IDA]
- platelet dense tubular network membrane [IDA]
- recycling endosome membrane [ISO]
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| ATXN1 EHD1 | Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods. | High | - | BioGRID | 2622166 |
Curated By
- BioGRID