BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
G6PDX
G28A, G6pd, Gpdx, RP23-436K3.17-003
glucose-6-phosphate dehydrogenase X-linked
GO Process (24)
GO Function (5)
GO Component (9)
Gene Ontology Biological Process
- NADP biosynthetic process [IDA, IMP]
- NADP metabolic process [ISO]
- NADPH regeneration [ISO]
- angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure [IMP]
- cellular response to oxidative stress [ISO]
- cholesterol biosynthetic process [ISO]
- cytokine production [IMP, ISO]
- erythrocyte development [IMP]
- erythrocyte maturation [ISO]
- glucose 6-phosphate metabolic process [ISO]
- glutathione metabolic process [IMP, ISO]
- interleukin-10 production [IMP]
- interleukin-12 production [IMP]
- negative regulation of protein glutathionylation [ISO]
- oxidation-reduction process [IMP, ISO]
- pentose biosynthetic process [IDA, IMP, ISO]
- pentose-phosphate shunt [ISO]
- pentose-phosphate shunt, oxidative branch [ISO]
- regulation of multicellular organism growth [IMP]
- regulation of neuron apoptotic process [ISO]
- response to ethanol [ISO]
- response to oxidative stress [IMP]
- ribose phosphate biosynthetic process [ISO]
- vasodilation by angiotensin involved in regulation of systemic arterial blood pressure [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID