BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
PRMT5
Jbp1, Skb1
protein arginine N-methyltransferase 5
GO Process (14)
GO Function (9)
GO Component (5)
Gene Ontology Biological Process
- circadian regulation of gene expression [IMP]
- endothelial cell activation [ISO]
- histone H4-R3 methylation [IMP]
- histone arginine methylation [IBA]
- negative regulation of cell differentiation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IMP]
- peptidyl-arginine N-methylation [ISO]
- peptidyl-arginine methylation [ISO]
- peptidyl-arginine methylation, to symmetrical-dimethyl arginine [IBA, ISO]
- positive regulation of oligodendrocyte differentiation [ISO]
- protein methylation [ISO]
- regulation of DNA methylation [ISO]
- regulation of ERK1 and ERK2 cascade [ISO]
- spliceosomal snRNP assembly [ISO]
Gene Ontology Molecular Function- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
- chromatin binding [IMP]
- core promoter sequence-specific DNA binding [IDA]
- histone-arginine N-methyltransferase activity [IBA]
- methyltransferase activity [ISO]
- protein binding [IPI]
- protein complex binding [ISO]
- protein-arginine omega-N symmetric methyltransferase activity [ISO]
- ribonucleoprotein complex binding [ISO]
- transcription corepressor activity [IMP]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID