BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
CLIC4
D0Jmb3, TU-74, mc3s5, mtCLIC, RP23-230K10.1
chloride intracellular channel 4 (mitochondrial)
GO Process (12)
GO Function (3)
GO Component (18)
Gene Ontology Biological Process
- angiogenesis [IMP]
- branching morphogenesis of an epithelial tube [IMP]
- cellular response to calcium ion [ISO]
- chloride transmembrane transport [ISO]
- chloride transport [ISO]
- endothelial cell morphogenesis [IMP]
- fertilization [IMP]
- keratinocyte differentiation [IMP, ISO]
- multicellular organism growth [IMP]
- negative regulation of cell migration [ISO]
- retina vasculature morphogenesis in camera-type eye [IMP]
- vacuolar acidification [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [ISO]
- cell [IMP]
- cell surface [ISO]
- cell-cell junction [ISO]
- centrosome [ISO]
- cytoplasm [ISO]
- cytosol [ISO]
- extracellular vesicular exosome [ISO]
- integral component of membrane [ISS]
- intracellular [IMP, ISO]
- microtubule cytoskeleton [ISO]
- microvillus [ISO]
- midbody [ISO]
- mitochondrion [IDA, ISO]
- nuclear matrix [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- vacuole [IMP]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID