BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
STOML2
0610038F01Rik, MSLP2, SLP-2, RP23-124L1.8
stomatin (Epb7.2)-like 2
GO Process (15)
GO Function (1)
GO Component (11)
Gene Ontology Biological Process
- CD4-positive, alpha-beta T cell activation [IMP]
- T cell receptor signaling pathway [ISO]
- cellular calcium ion homeostasis [ISO]
- interleukin-2 production [IMP]
- lipid localization [IMP]
- mitochondrial ATP synthesis coupled proton transport [IMP, ISO]
- mitochondrial calcium ion transport [ISO]
- mitochondrial protein processing [IMP]
- mitochondrion localization [IMP]
- mitochondrion organization [ISO]
- positive regulation of cardiolipin metabolic process [ISO]
- positive regulation of mitochondrial DNA replication [ISO]
- positive regulation of mitochondrial membrane potential [ISO]
- protein oligomerization [ISO]
- stress-induced mitochondrial fusion [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- T cell receptor complex [ISO]
- actin cytoskeleton [ISO]
- cell [IMP]
- extrinsic component of plasma membrane [IDA, ISO]
- immunological synapse [ISO]
- intracellular [IMP]
- membrane raft [ISO]
- mitochondrial inner membrane [IDA, ISO]
- mitochondrial intermembrane space [ISO]
- mitochondrion [IDA, IMP]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID