Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.

Zhang S, Williamson NA, Bogoyevitch MA

Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]

Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]

Throughput

  • High Throughput

Additional Notes

  • ataxin-1[85Q]
  • in the presence of Arsenite

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
ATXN1 IPO5
Proximity Label-MS
Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

High-BioGRID
2622303

Curated By

  • BioGRID