BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
ATP1A2
AW060654, Atpa-3, mKIAA0778
ATPase, Na+/K+ transporting, alpha 2 polypeptide
GO Process (30)
GO Function (6)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- ATP hydrolysis coupled proton transport [ISO]
- adult locomotory behavior [IMP]
- cellular potassium ion homeostasis [ISO]
- cellular response to mechanical stimulus [ISO]
- cellular response to steroid hormone stimulus [IDA, ISO]
- cellular sodium ion homeostasis [ISO]
- locomotion [IMP]
- negative regulation of calcium ion transmembrane transport [ISO]
- negative regulation of calcium:sodium antiporter activity [ISO]
- negative regulation of cytosolic calcium ion concentration [IMP]
- negative regulation of heart contraction [IMP]
- negative regulation of striated muscle contraction [IMP]
- neurotransmitter uptake [IMP]
- potassium ion import [ISO]
- potassium ion transport [ISO]
- regulation of blood pressure [IGI]
- regulation of cardiac muscle cell contraction [ISO]
- regulation of muscle contraction [IMP]
- regulation of respiratory gaseous exchange by neurological system process [IMP]
- regulation of smooth muscle contraction [IMP]
- regulation of striated muscle contraction [IMP]
- regulation of the force of heart contraction [IMP]
- regulation of vasoconstriction [IGI, IMP]
- response to glycoside [IMP, ISO]
- response to nicotine [ISO]
- sodium ion export from cell [ISO]
- sodium ion transmembrane transport [ISO]
- sodium ion transport [ISO]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
Curated By
- BioGRID