BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
MYO1C
C80397, MMIb, MYO1E, NMI, mm1beta, myr2, RP23-136K12.2
myosin IC
GO Process (9)
GO Function (8)
GO Component (15)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- positive regulation of cell migration [ISO]
- positive regulation of cell migration by vascular endothelial growth factor signaling pathway [ISO]
- positive regulation of protein targeting to membrane [ISO]
- positive regulation of vascular endothelial growth factor signaling pathway [ISO]
- protein targeting [ISO]
- protein targeting to membrane [ISO]
- regulation of tight junction assembly [ISO]
- transport [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- basal plasma membrane [ISO]
- brush border [IDA]
- cytoplasm [ISO]
- extracellular vesicular exosome [ISO]
- filamentous actin [ISO]
- lateral plasma membrane [ISO]
- membrane [ISO, TAS]
- membrane raft [ISO]
- microvillus [ISO]
- mitochondrion [ISO]
- myosin I complex [IDA]
- nucleoplasm [ISO]
- nucleus [ISO]
- plasma membrane [ISO]
- stereocilium [IDA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID