BAIT
ATXN1
2900016G23Rik, Atx1, C85907, ENSMUSG00000074917, Gm10786, Sca1
ataxin 1
GO Process (11)
GO Function (8)
GO Component (7)
Gene Ontology Biological Process
- adult locomotory behavior [IMP]
- lung alveolus development [IGI]
- negative regulation of insulin-like growth factor receptor signaling pathway [IMP]
- negative regulation of phosphorylation [IMP]
- negative regulation of transcription from RNA polymerase II promoter [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- nuclear export [ISO]
- positive regulation of glial cell proliferation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- regulation of excitatory postsynaptic membrane potential [IMP]
- visual learning [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Mus musculus
PREY
GSK3B
7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3
glycogen synthase kinase 3 beta
GO Process (56)
GO Function (17)
GO Component (19)
Gene Ontology Biological Process
- ER overload response [IDA, ISO]
- Wnt signaling pathway [IGI]
- axonogenesis [IGI]
- canonical Wnt signaling pathway [IDA, ISO]
- canonical Wnt signaling pathway involved in positive regulation of apoptotic process [IMP]
- cell migration [IGI]
- cell proliferation [TAS]
- cellular response to interleukin-3 [IDA]
- cellular response to mechanical stimulus [ISO]
- circadian rhythm [IMP]
- cytoskeleton organization [TAS]
- epithelial to mesenchymal transition [ISO]
- establishment of cell polarity [ISO]
- establishment or maintenance of cell polarity [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- fat cell differentiation [IDA]
- glycogen metabolic process [ISO]
- hippocampus development [ISO]
- hypermethylation of CpG island [IMP]
- intracellular signal transduction [ISO]
- myoblast fusion [IDA, IGI]
- myotube differentiation [IGI]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of NFAT protein import into nucleus [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cardiac muscle hypertrophy [IDA]
- negative regulation of dendrite morphogenesis [ISO]
- negative regulation of neuron maturation [IGI]
- negative regulation of neuron projection development [IGI, IMP]
- negative regulation of protein binding [ISO]
- negative regulation of protein complex assembly [ISO]
- organ morphogenesis [IMP]
- peptidyl-serine phosphorylation [IDA, ISO]
- phosphorylation [IMP]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of axon extension [IGI]
- positive regulation of cell-matrix adhesion [ISO]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [IDA]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein complex assembly [ISO]
- positive regulation of protein export from nucleus [ISO]
- positive regulation of stem cell differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein export from nucleus [IDA]
- protein localization to microtubule [IGI]
- protein phosphorylation [IDA, IGI, IMP, ISO, ISS]
- re-entry into mitotic cell cycle [IDA]
- regulation of gene expression by genetic imprinting [IMP]
- regulation of microtubule-based process [IDA, ISO]
- regulation of neuron projection development [IGI]
- regulation of neuronal synaptic plasticity [ISO]
- superior temporal gyrus development [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [IPI, ISO]
- integrin binding [ISO]
- ionotropic glutamate receptor binding [ISO]
- kinase activity [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, IMP, ISO, ISS]
- tau protein binding [ISO]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ATP binding [ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [IPI, ISO]
- integrin binding [ISO]
- ionotropic glutamate receptor binding [ISO]
- kinase activity [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, IMP, ISO, ISS]
- tau protein binding [ISO]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- beta-catenin destruction complex [IDA, ISO]
- cell body [IDA]
- centrosome [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- dendritic shaft [IDA]
- dendritic spine [ISO]
- growth cone [IDA]
- membrane [ISO]
- membrane raft [ISO]
- membrane-bounded organelle [IDA]
- neuronal cell body [IDA]
- neuronal postsynaptic density [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [IDA]
Mus musculus
Affinity Capture-MS
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.
Publication
Complementary proteomics strategies capture an ataxin-1 interactome in Neuro-2a cells.
Ataxin-1 mutation, arising from a polyglutamine (polyQ) tract expansion, is the underlying genetic cause of the late-onset neurodegenerative disease Spinocerebellar ataxia type 1 (SCA1). To identify protein partners of polyQ-ataxin-1 in neuronal cells under control or stress conditions, here we report our complementary proteomics strategies of proximity-dependent biotin identification (BioID) and affinity purification (via GFP-Trap pulldown) in Neuro-2a cells expressing ... [more]
Sci Data Dec. 20, 2017; 5(1);180262 [Pubmed: 30457570]
Throughput
- High Throughput
Additional Notes
- ataxin-1[85Q]
- in the presence of Arsenite
Curated By
- BioGRID