Proximity Label-MS

An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.

Publication

USP7 Regulates Cytokinesis through FBXO38 and KIF20B.

Georges A, Coyaud E, Marcon E, Greenblatt J, Raught B, Frappier L

The ubiquitin specific protease 7 (USP7 or HAUSP) is known to regulate a variety of cellular processes by binding and deubiquitylating specific target proteins. To gain a more comprehensive understanding of its interactions and functions, we used affinity purification coupled to mass spectrometry to profile USP7 interactions. This revealed a novel interaction with FBXO38, a poorly characterized F-box protein. We ... [more]

Sci Rep Feb. 25, 2019; 9(1);2724 [Pubmed: 30804394]

Throughput

  • High Throughput

Curated By

  • BioGRID