BAIT
TRP53
Tp53, bbl, bfy, bhy, p44, p53, RP23-56I20.1
transformation related protein 53
GO Process (100)
GO Function (32)
GO Component (17)
Gene Ontology Biological Process
- B cell lineage commitment [IMP]
- DNA damage response, signal transduction by p53 class mediator [IDA, IGI, ISO]
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IGI]
- DNA damage response, signal transduction by p53 class mediator resulting in transcription of p21 class mediator [IBA, ISO]
- DNA strand renaturation [ISO]
- ER overload response [ISO]
- T cell differentiation in thymus [IGI]
- T cell lineage commitment [IMP]
- T cell proliferation involved in immune response [IGI]
- apoptotic process [IDA, IGI]
- cell aging [IGI, ISO]
- cell cycle arrest [ISO]
- cellular protein localization [ISO]
- cellular response to DNA damage stimulus [IDA, IMP, ISO]
- cellular response to UV [IGI]
- cellular response to glucose starvation [ISO]
- cellular response to ionizing radiation [IGI, ISO]
- central nervous system development [IGI]
- cerebellum development [IDA]
- chromatin assembly [ISO]
- chromosome organization [IGI]
- determination of adult lifespan [IMP]
- double-strand break repair [IMP]
- embryo development ending in birth or egg hatching [IGI]
- embryonic organ development [IGI]
- gastrulation [IGI]
- in utero embryonic development [IGI]
- intrinsic apoptotic signaling pathway by p53 class mediator [IDA, IGI, ISO]
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator [IGI, IMP, ISO]
- mitochondrial DNA repair [IMP]
- mitotic G1 DNA damage checkpoint [IMP, ISO]
- mitotic cell cycle arrest [IDA]
- multicellular organism growth [IGI]
- multicellular organismal development [ISO]
- necroptotic process [IGI]
- negative regulation of DNA biosynthetic process [ISO]
- negative regulation of DNA replication [IDA, IMP]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cell growth [ISO]
- negative regulation of cell proliferation [IGI, IMP]
- negative regulation of fibroblast proliferation [IMP, ISO]
- negative regulation of macromitophagy [IMP]
- negative regulation of mitotic cell cycle [IGI]
- negative regulation of neuroblast proliferation [IGI]
- negative regulation of proteolysis [IMP]
- negative regulation of reactive oxygen species metabolic process [IMP]
- negative regulation of smooth muscle cell proliferation [ISO]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IGI, IMP, ISO]
- negative regulation of transcription, DNA-templated [IMP, ISO]
- negative regulation of transforming growth factor beta receptor signaling pathway [IMP]
- neuron apoptotic process [IGI, IMP]
- nucleotide-excision repair [ISO]
- oligodendrocyte apoptotic process [ISO]
- oxidative stress-induced premature senescence [ISO]
- positive regulation of apoptotic process [IGI, IMP, ISO]
- positive regulation of cardiac muscle cell apoptotic process [IMP]
- positive regulation of cell aging [IMP]
- positive regulation of cell cycle [ISO]
- positive regulation of cell cycle arrest [ISO]
- positive regulation of histone deacetylation [IDA]
- positive regulation of intrinsic apoptotic signaling pathway [ISO]
- positive regulation of leukocyte migration [ISO]
- positive regulation of mitochondrial membrane permeability [IGI]
- positive regulation of neuron apoptotic process [IDA, IGI, IMP]
- positive regulation of peptidyl-tyrosine phosphorylation [IMP]
- positive regulation of protein oligomerization [ISO]
- positive regulation of reactive oxygen species metabolic process [ISO]
- positive regulation of release of cytochrome c from mitochondria [ISO]
- positive regulation of thymocyte apoptotic process [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IBA, IDA, IGI, IMP, ISO]
- positive regulation of transcription, DNA-templated [IDA, IGI, ISO]
- protein complex assembly [ISO]
- protein import into nucleus, translocation [IDA]
- protein localization [ISO]
- rRNA transcription [IGI]
- regulation of apoptotic process [ISO]
- regulation of catalytic activity [IDA]
- regulation of cell cycle [IGI, IMP]
- regulation of cell proliferation [IGI, IMP]
- regulation of fibroblast apoptotic process [IGI]
- regulation of intracellular pH [ISO]
- regulation of intrinsic apoptotic signaling pathway by p53 class mediator [IMP]
- regulation of mitochondrial membrane permeability involved in apoptotic process [IGI]
- regulation of neuron apoptotic process [IGI]
- regulation of thymocyte apoptotic process [IGI]
- regulation of tissue remodeling [IMP]
- regulation of transcription from RNA polymerase II promoter [IDA]
- regulation of transcription, DNA-templated [IDA, ISO]
- release of cytochrome c from mitochondria [IDA, IGI]
- replicative senescence [ISO]
- response to UV [IDA, IMP]
- response to X-ray [IDA]
- response to drug [IDA]
- response to gamma radiation [IDA, IMP, ISO]
- response to ischemia [IMP]
- response to oxidative stress [IGI, IMP]
- response to salt stress [IGI]
- somitogenesis [IGI]
- transcription from RNA polymerase II promoter [IGI]
- transforming growth factor beta receptor signaling pathway [IGI]
Gene Ontology Molecular Function- ATP binding [ISO]
- DNA binding [IDA, ISO]
- MDM2/MDM4 family protein binding [IPI, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IGI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- chaperone binding [ISO]
- chromatin binding [IDA, ISO]
- copper ion binding [ISO]
- damaged DNA binding [IBA]
- double-stranded DNA binding [IBA]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase regulator activity [IDA]
- identical protein binding [ISO]
- p53 binding [IBA]
- protease binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- receptor tyrosine kinase binding [ISO]
- sequence-specific DNA binding [IBA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription cofactor binding [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ATP binding [ISO]
- DNA binding [IDA, ISO]
- MDM2/MDM4 family protein binding [IPI, ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [IDA, IGI]
- RNA polymerase II core promoter sequence-specific DNA binding [IDA]
- RNA polymerase II transcription factor binding [ISO]
- RNA polymerase II transcription regulatory region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription [ISO]
- chaperone binding [ISO]
- chromatin binding [IDA, ISO]
- copper ion binding [ISO]
- damaged DNA binding [IBA]
- double-stranded DNA binding [IBA]
- enzyme binding [ISO]
- histone acetyltransferase binding [ISO]
- histone deacetylase regulator activity [IDA]
- identical protein binding [ISO]
- p53 binding [IBA]
- protease binding [ISO]
- protein C-terminus binding [ISO]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein heterodimerization activity [ISO]
- protein kinase binding [ISO]
- protein phosphatase 2A binding [ISO]
- protein phosphatase binding [ISO]
- receptor tyrosine kinase binding [ISO]
- sequence-specific DNA binding [IBA, ISO]
- sequence-specific DNA binding transcription factor activity [IDA, ISO]
- transcription cofactor binding [ISO]
- transcription factor binding [ISO]
- transcription regulatory region DNA binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- PML body [ISO]
- chromatin [IBA, ISO]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- intracellular [IGI]
- mitochondrial matrix [IDA]
- mitochondrion [IBA, IDA, IGI, ISO]
- nuclear body [ISO]
- nuclear chromatin [ISO]
- nuclear matrix [ISO]
- nucleolus [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- protein complex [ISO]
- replication fork [IDA]
- transcription factor TFIID complex [ISO]
- transcription factor complex [IBA, ISO]
Mus musculus
PREY
IRS1
G972R, IRS-1
insulin receptor substrate 1
GO Process (28)
GO Function (10)
GO Component (9)
Gene Ontology Biological Process
- cellular response to insulin stimulus [IDA, ISO]
- insulin receptor signaling pathway [IBA, ISO]
- insulin secretion [IMP]
- insulin-like growth factor receptor signaling pathway [ISO]
- lipid catabolic process [IMP]
- mammary gland development [IGI]
- negative regulation of insulin receptor signaling pathway [ISO]
- negative regulation of insulin secretion [ISO]
- negative regulation of somatostatin secretion [ISO]
- phosphatidylinositol 3-kinase signaling [ISO]
- positive regulation of cell migration [IGI]
- positive regulation of fatty acid beta-oxidation [ISO]
- positive regulation of glucagon secretion [ISO]
- positive regulation of glucose import [ISO]
- positive regulation of glucose import in response to insulin stimulus [ISO]
- positive regulation of glucose metabolic process [ISO]
- positive regulation of glycogen biosynthetic process [ISO]
- positive regulation of insulin receptor signaling pathway [ISO]
- positive regulation of mesenchymal cell proliferation [IGI]
- positive regulation of phosphatidylinositol 3-kinase activity [IMP, ISO]
- positive regulation of phosphorylation [ISO]
- protein heterooligomerization [ISO]
- protein kinase B signaling [IGI]
- protein localization to nucleus [IDA, IMP]
- regulation of gene expression [IMP]
- response to caffeine [ISO]
- response to insulin [ISO]
- response to peptide hormone [ISO]
Gene Ontology Molecular Function- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
- SH2 domain binding [ISO]
- insulin receptor binding [IBA, ISO]
- insulin-like growth factor receptor binding [ISO]
- phosphatidylinositol 3-kinase binding [IDA, ISO]
- protein binding [IPI]
- protein domain specific binding [ISO]
- protein kinase C binding [ISO]
- protein kinase binding [IDA, ISO]
- signal transducer activity [IBA]
- transmembrane receptor protein tyrosine kinase adaptor activity [ISO]
Gene Ontology Cellular Component
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Hyperglycemia induces vascular smooth muscle cell dedifferentiation by suppressing insulin receptor substrate-1-mediated p53/KLF4 complex stabilization.
Hyperglycemia and insulin resistance accelerate atherosclerosis by an unclear mechanism. The two factors down-regulate insulin receptor substrate-1 (IRS-1), an intermediary of the insulin/IGF-I signaling system. We previously reported that IRS-1 down-regulation leads to vascular smooth muscle cell (VSMC) dedifferentiation and that IRS-1 deletion from VSMCs in normoglycemic mice replicates this response. However, we did not determine IRS-1's role in mediating ... [more]
J. Biol. Chem. Dec. 15, 2018; 294(7);2407-2421 [Pubmed: 30578299]
Throughput
- Low Throughput
Curated By
- BioGRID