SNAI2
Gene Ontology Biological Process
- Notch signaling pathway [IMP]
- canonical Wnt signaling pathway [IMP]
- cell migration involved in endocardial cushion formation [ISS]
- cellular response to epidermal growth factor stimulus [IDA]
- desmosome disassembly [IMP]
- epithelial to mesenchymal transition [IMP]
- epithelial to mesenchymal transition involved in endocardial cushion formation [ISS]
- epithelium development [ISS]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IMP]
- negative regulation of anoikis [IMP]
- negative regulation of canonical Wnt signaling pathway [IDA, IMP]
- negative regulation of catenin import into nucleus [IDA]
- negative regulation of cell adhesion mediated by integrin [IC]
- negative regulation of cell-cell adhesion by negative regulation of transcription from RNA polymerase II promoter [IMP]
- negative regulation of chondrocyte differentiation [IMP]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISS]
- negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage [IMP]
- negative regulation of keratinocyte proliferation [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA, IMP]
- negative regulation of vitamin D biosynthetic process [IDA]
- negative regulation of vitamin D receptor signaling pathway [IDA]
- neural crest cell development [IMP]
- osteoblast differentiation [IEP]
- pigmentation [IMP]
- positive regulation of cell migration [IMP]
- regulation of chemokine production [IMP]
- regulation of osteoblast differentiation [IMP]
- regulation of tight junction assembly [IMP]
- sensory perception of sound [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
USP5
Gene Ontology Biological Process
Gene Ontology Molecular Function
Co-localization
Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments.
Publication
USP5 promotes epithelial-mesenchymal transition by stabilizing SLUG in hepatocellular carcinoma.
Rationale: The role of SLUG in epithelial-mesenchymal transition during tumor progression has been thoroughly studied, but its precise regulation remains poorly explored. Methods: The affinity purification, mass spectrometry and CO-IP were performed to identify the interaction between SLUG and ubiquitin-specific protease 5 (USP5). Cycloheximide chase assays and deubiquitination assays confirmed that the effect of USP5 on the deubiquitin of SLUG. ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| SNAI2 USP5 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | High | - | BioGRID | - | |
| SNAI2 USP5 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP5 SNAI2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| USP5 SNAI2 | Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex. | Low | - | BioGRID | - |
Curated By
- BioGRID