An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

SRPK2 phosphorylates tau and mediates the cognitive defects in Alzheimer's disease.

Hong Y, Chan CB, Kwon IS, Li X, Song M, Lee HP, Liu X, Sompol P, Jin P, Lee HG, Yu SP, Ye K

Serine-arginine protein kinases 2 (SRPK2) is a cell cycle-regulated kinase that phosphorylates serine/arginine domain-containing proteins and mediates pre-mRNA splicing with unclear function in neurons. Here, we show that SRPK2 phosphorylates tau on S214, suppresses tau-dependent microtubule polymerization, and inhibits axonal elongation in neurons. Depletion of SRPK2 in dentate gyrus inhibits tau phosphorylation in APP/PS1 mouse and alleviates the impaired cognitive ... [more]

J. Neurosci. Nov. 28, 2012; 32(48);17262-72 [Pubmed: 23197718]

Throughput

Low Throughput

Curated By

BioGRID

Interaction Summary

MAPT

Gene Ontology Biological Process

Gene Ontology Molecular Function

SH3 domain binding [ISO]apolipoprotein binding [ISO]enzyme binding [ISO]lipoprotein particle binding [ISO]microtubule binding [IDA, ISO]protein binding [IPI]protein complex binding [ISO]protein domain specific binding [ISO]protein kinase binding [IPI, ISO]protein phosphatase 2A binding [ISO]

Gene Ontology Cellular Component

SRPK1