BAIT
SLC8A1
AI852629, AV344025, D930008O12Rik, Ncx1
solute carrier family 8 (sodium/calcium exchanger), member 1
GO Process (38)
GO Function (6)
GO Component (17)
Gene Ontology Biological Process
- calcium ion export [IMP, ISO]
- calcium ion homeostasis [IMP]
- calcium ion import [IMP, ISO]
- calcium ion transmembrane transport [IMP, ISO]
- calcium ion transport [IDA, IMP]
- calcium ion transport into cytosol [IMP, ISO]
- cardiac muscle cell development [IMP]
- cardiac muscle contraction [IGI]
- cell communication by electrical coupling involved in cardiac conduction [ISO]
- cellular calcium ion homeostasis [ISO]
- cellular response to cAMP [ISO]
- cellular response to caffeine [IMP]
- cellular response to reactive oxygen species [ISO]
- cellular sodium ion homeostasis [ISO]
- embryonic heart tube development [IMP]
- embryonic placenta development [IMP]
- heart morphogenesis [IGI]
- muscle fiber development [IGI]
- negative regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of fibroblast migration [ISO]
- positive regulation of the force of heart contraction [ISO]
- post-embryonic development [IGI]
- regulation of calcium ion transport [ISO]
- regulation of cardiac muscle cell membrane potential [TAS]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [ISO]
- regulation of heart rate [IMP]
- regulation of sodium ion transport [ISO]
- regulation of the force of heart contraction [IMP]
- relaxation of smooth muscle [IMP]
- response to ATP [ISO]
- response to hypoxia [ISO]
- response to muscle stretch [ISO]
- sodium ion export [IMP, ISO]
- sodium ion import [IMP, ISO]
- sodium ion transmembrane transport [IDA]
- sodium ion transport [IDA, ISO]
- vascular smooth muscle contraction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA, ISO]
- Z disc [ISO]
- basolateral plasma membrane [IDA]
- cell [IMP]
- cell projection [ISO]
- cytosol [IMP]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- integral component of membrane [TAS]
- integral component of plasma membrane [ISO]
- intercalated disc [ISO]
- intracellular [IMP]
- membrane [IDA]
- microtubule [ISO]
- mitochondrion [ISO]
- plasma membrane [IDA, ISO]
- sarcolemma [IDA, ISO]
Mus musculus
PREY
GPX1
GSHPx, GSHPx-1
glutathione peroxidase 1
GO Process (52)
GO Function (5)
GO Component (6)
Gene Ontology Biological Process
- UV protection [ISO]
- aging [IEP]
- angiogenesis involved in wound healing [ISO]
- apoptotic process [ISO]
- blood vessel endothelial cell migration [ISO]
- cell proliferation [ISO]
- cell redox homeostasis [ISO]
- endothelial cell development [ISO]
- fat cell differentiation [ISO]
- glutathione metabolic process [IDA, ISO]
- heart contraction [ISO]
- hydrogen peroxide catabolic process [IDA, ISO]
- interaction with symbiont [ISO]
- intrinsic apoptotic signaling pathway in response to oxidative stress [ISO]
- lipid metabolic process [ISO]
- myoblast proliferation [ISO]
- myotube differentiation [ISO]
- negative regulation of apoptotic process [ISO]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [ISO]
- negative regulation of extrinsic apoptotic signaling pathway via death domain receptors [ISO]
- negative regulation of inflammatory response to antigenic stimulus [ISO]
- negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway [ISO]
- negative regulation of release of cytochrome c from mitochondria [ISO]
- positive regulation of protein kinase B signaling [ISO]
- protein oxidation [ISO]
- regulation of gene expression, epigenetic [ISO]
- regulation of mammary gland epithelial cell proliferation [ISO]
- regulation of neuron apoptotic process [ISO]
- regulation of proteasomal protein catabolic process [ISO]
- response to estradiol [IEP]
- response to folic acid [IEP]
- response to gamma radiation [ISO]
- response to glucose [IEP]
- response to hormone [IEP]
- response to hydrogen peroxide [ISO]
- response to hydroperoxide [ISO]
- response to lipid hydroperoxide [IMP]
- response to nicotine [IEP]
- response to organic cyclic compound [IEP]
- response to oxidative stress [ISO, TAS]
- response to reactive oxygen species [ISO]
- response to selenium ion [IEP, ISO]
- response to symbiotic bacterium [ISO]
- response to toxic substance [ISO]
- response to wounding [ISO]
- response to xenobiotic stimulus [ISO]
- sensory perception of sound [ISO]
- skeletal muscle fiber development [ISO]
- skeletal muscle tissue regeneration [ISO]
- temperature homeostasis [ISO]
- triglyceride metabolic process [ISO]
- vasodilation [ISO]
Gene Ontology Molecular Function
Rattus norvegicus
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Mapping the in vitro interactome of cardiac sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1).
The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this study, we used affinity purification coupled with MS in rat left ... [more]
Proteomics Sep. 01, 2017; 17(17-18); [Pubmed: 28755400]
Throughput
- High Throughput
Curated By
- BioGRID