BAIT
SLC8A1
AI852629, AV344025, D930008O12Rik, Ncx1
solute carrier family 8 (sodium/calcium exchanger), member 1
GO Process (38)
GO Function (6)
GO Component (17)
Gene Ontology Biological Process
- calcium ion export [IMP, ISO]
- calcium ion homeostasis [IMP]
- calcium ion import [IMP, ISO]
- calcium ion transmembrane transport [IMP, ISO]
- calcium ion transport [IDA, IMP]
- calcium ion transport into cytosol [IMP, ISO]
- cardiac muscle cell development [IMP]
- cardiac muscle contraction [IGI]
- cell communication by electrical coupling involved in cardiac conduction [ISO]
- cellular calcium ion homeostasis [ISO]
- cellular response to cAMP [ISO]
- cellular response to caffeine [IMP]
- cellular response to reactive oxygen species [ISO]
- cellular sodium ion homeostasis [ISO]
- embryonic heart tube development [IMP]
- embryonic placenta development [IMP]
- heart morphogenesis [IGI]
- muscle fiber development [IGI]
- negative regulation of cytosolic calcium ion concentration [IMP]
- positive regulation of cytosolic calcium ion concentration [ISO]
- positive regulation of fibroblast migration [ISO]
- positive regulation of the force of heart contraction [ISO]
- post-embryonic development [IGI]
- regulation of calcium ion transport [ISO]
- regulation of cardiac muscle cell membrane potential [TAS]
- regulation of cardiac muscle contraction by regulation of the release of sequestered calcium ion [ISO]
- regulation of heart rate [IMP]
- regulation of sodium ion transport [ISO]
- regulation of the force of heart contraction [IMP]
- relaxation of smooth muscle [IMP]
- response to ATP [ISO]
- response to hypoxia [ISO]
- response to muscle stretch [ISO]
- sodium ion export [IMP, ISO]
- sodium ion import [IMP, ISO]
- sodium ion transmembrane transport [IDA]
- sodium ion transport [IDA, ISO]
- vascular smooth muscle contraction [IMP]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- T-tubule [IDA, ISO]
- Z disc [ISO]
- basolateral plasma membrane [IDA]
- cell [IMP]
- cell projection [ISO]
- cytosol [IMP]
- dendritic shaft [ISO]
- dendritic spine [ISO]
- integral component of membrane [TAS]
- integral component of plasma membrane [ISO]
- intercalated disc [ISO]
- intracellular [IMP]
- membrane [IDA]
- microtubule [ISO]
- mitochondrion [ISO]
- plasma membrane [IDA, ISO]
- sarcolemma [IDA, ISO]
Mus musculus
PREY
HSPA1A
HSP72, Hsp70-1, Hspa1, Hspa1b
heat shock 70kD protein 1A
GO Process (24)
GO Function (15)
GO Component (22)
Gene Ontology Biological Process
- ATP catabolic process [ISO]
- DNA repair [ISO]
- binding of sperm to zona pellucida [ISO]
- cellular heat acclimation [ISO]
- cellular response to heat [ISO]
- defense response [IDA]
- mRNA catabolic process [ISO, ISS]
- negative regulation of apoptotic process [IDA, ISO]
- negative regulation of cell death [ISO]
- negative regulation of cell growth [ISO, ISS]
- negative regulation of cell proliferation [ISO, ISS]
- negative regulation of cysteine-type endopeptidase activity involved in apoptotic process [IDA, ISO]
- negative regulation of extrinsic apoptotic signaling pathway in absence of ligand [ISO]
- negative regulation of inclusion body assembly [ISO]
- negative regulation of myeloid cell apoptotic process [IDA]
- negative regulation of protein ubiquitination [ISO]
- negative regulation of vasoconstriction [IMP]
- positive regulation of T cell mediated cytotoxicity [IDA]
- positive regulation of erythrocyte differentiation [ISO]
- protein refolding [ISO]
- regulation of cell death [ISO]
- response to heat [ISO]
- response to unfolded protein [IMP, ISO, ISS]
- telomere maintenance [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [IPI]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
- ATP binding [ISO]
- ATPase activity [ISO]
- ATPase activity, coupled [ISO]
- G-protein coupled receptor binding [ISO]
- NF-kappaB binding [IPI]
- double-stranded RNA binding [ISO]
- enzyme binding [ISO]
- heat shock protein binding [ISO]
- poly(A) RNA binding [ISO]
- protease binding [IPI]
- protein N-terminus binding [ISO]
- protein binding [IPI]
- protein binding involved in protein folding [ISO]
- ubiquitin protein ligase binding [ISO]
- unfolded protein binding [ISO]
Gene Ontology Cellular Component
- COP9 signalosome [ISO]
- aggresome [ISO]
- apical plasma membrane [IDA]
- basolateral plasma membrane [IDA]
- blood microparticle [ISO]
- cell body [ISO]
- centriole [ISO]
- cytoplasm [ISO, ISS]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- inclusion body [ISO]
- intracellular [ISO]
- mitochondrion [ISO]
- nuclear speck [ISO, ISS]
- nucleus [IDA, ISO, ISS]
- perinuclear region of cytoplasm [ISO, ISS]
- protein complex [IDA]
- ribonucleoprotein complex [ISO]
- ubiquitin ligase complex [ISO]
- vesicle [ISO]
- zona pellucida receptor complex [ISO]
Rattus norvegicus
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Mapping the in vitro interactome of cardiac sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1).
The sodium (Na+ )-calcium (Ca2+ ) exchanger 1 (NCX1) is an antiporter membrane protein encoded by the SLC8A1 gene. In the heart, it maintains cytosolic Ca2+ homeostasis, serving as the primary mechanism for Ca2+ extrusion during relaxation. Dysregulation of NCX1 is observed in end-stage human heart failure. In this study, we used affinity purification coupled with MS in rat left ... [more]
Proteomics Sep. 01, 2017; 17(17-18); [Pubmed: 28755400]
Throughput
- High Throughput
Curated By
- BioGRID