A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.

Publication

Fission yeast cells overproducing HSET/KIFC1 provides a useful tool for identification and evaluation of human kinesin-14 inhibitors.

Yukawa M, Yamauchi T, Kurisawa N, Ahmed S, Kimura KI, Toda T

Many human cancer cells contain more than two centrosomes, yet these cancer cells can form pseudo-bipolar spindles through the mechanism, called centrosome clustering, and survive, instead of committing lethal multipolar mitoses. Kinesin-14/HSET, a minus end-directed motor, plays a crucial role in centrosome clustering. Accordingly, HSET is deemed to be a promising chemotherapeutic target to selectively kill cancer cells. Recently, three ... [more]

Fungal Genet. Biol. Dec. 01, 2017; 116();33-41 [Pubmed: 29684553]

Throughput

Low Throughput

Ontology Terms

spindle morphology (APO:0000213)

Additional Notes

Figure 1
Overexpression of cut7 rescues spindle structure defect caused by pkl1 overexpression

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
CUT7 PKL1	Co-fractionation Co-fractionation Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.	Olmsted ZT (2014)	Low	-	BioGRID	-
PKL1 CUT7	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Rincon SA (2017)	Low	-	PomBase	-
PKL1 CUT7	Dosage Rescue Dosage Rescue A genetic interaction is inferred when over expression or increased dosage of one gene rescues the lethality or growth defect of a strain that is mutated or deleted for another gene.	Yukawa M (2018)	Low	-	BioGRID	2623440
PKL1 CUT7	Phenotypic Enhancement Phenotypic Enhancement A genetic interaction is inferred when mutation or overexpression of one gene results in enhancement of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Olmsted ZT (2014)	Low	-	BioGRID	2333097
PKL1 CUT7	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Syrovatkina V (2015)	Low	-	BioGRID	1429689
PKL1 CUT7	Phenotypic Suppression Phenotypic Suppression A genetic interaction is inferred when mutation or over expression of one gene results in suppression of any phenotype (other than lethality/growth defect) associated with mutation or over expression of another gene.	Yukawa M (2018)	Low	-	PomBase	-
PKL1 CUT7	Protein-peptide Protein-peptide An interaction is detected between a protein and a peptide derived from an interaction partner. This includes phage display experiments.	Olmsted ZT (2014)	Low	-	BioGRID	-
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Olmsted ZT (2013)	Low	-	BioGRID	823165
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Olmsted ZT (2014)	Low	-	BioGRID	2333090
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Pidoux AL (1996)	Low	-	BioGRID	246684
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Rodriguez AS (2008)	Low	-	BioGRID	298943
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Takeda A (2019)	Low	-	BioGRID	2609727
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Troxell CL (2001)	Low	-	BioGRID	246508
CUT7 PKL1	Synthetic Rescue Synthetic Rescue A genetic interaction is inferred when mutations or deletions of one gene rescues the lethality or growth defect of a strain mutated or deleted for another gene.	Yukawa M (2019)	Low	-	PomBase	-

Curated By

BioGRID

Interaction Summary

PKL1

Gene Ontology Biological Process

Gene Ontology Molecular Function

ATPase activity [IDA]gamma-tubulin binding [IPI]microtubule binding [IDA]minus-end-directed microtubule motor activity [IDA]

Gene Ontology Cellular Component

CUT7