Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Clustering of neuronal potassium channels is independent of their interaction with PSD-95.

Rasband MN, Park EW, Zhen D, Arbuckle MI, Poliak S, Peles E, Grant SG, Trimmer JS

Voltage-dependent potassium channels regulate membrane excitability and cell-cell communication in the mammalian nervous system, and are found highly localized at distinct neuronal subcellular sites. Kv1 (mammalian Shaker family) potassium channels and the neurexin Caspr2, both of which contain COOH-terminal PDZ domain binding peptide motifs, are found colocalized at high density at juxtaparanodes flanking nodes of Ranvier of myelinated axons. The ... [more]

J. Cell Biol. Nov. 25, 2002; 159(4);663-72 [Pubmed: 12438413]

Throughput

  • Low Throughput

Curated By

  • BioGRID