BAIT

SNAP25

SNAP-25B, SNAP-25a
synaptosomal-associated protein 25
GO Process (17)
GO Function (9)
GO Component (28)
Rattus norvegicus
PREY

ZWINT

Sip30, Zwint-1
ZW10 interacting kinetochore protein
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Identification of a novel SNAP25 interacting protein (SIP30).

Lee HK, Safieddine S, Petralia RS, Wenthold RJ

Soluble N -ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), including synaptosome-associated proteins of 25 kDa (SNAP25), syntaxins, and vesicle-associated membrane proteins (VAMP), are essential for regulated exocytosis of synaptic vesicles in neurotransmission. We identified a cDNA coding for a novel protein of 266 amino acids that we have named SIP30 (S NAP25 interacting protein of 30 kDa). SIP30 is expressed abundantly ... [more]

J. Neurochem. Jun. 01, 2002; 81(6);1338-47 [Pubmed: 12068081]

Throughput

  • Low Throughput

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
SNAP25 ZWINT
Reconstituted Complex
Reconstituted Complex

An interaction is detected between purified proteins in vitro.

Low-BioGRID
-

Curated By

  • BioGRID