Interaction inferred from the presence of two or more protein subunits in a partially purified protein preparation. If co-fractionation is demonstrated between 3 or more proteins, then add them as a complex.

Publication

Rewiring of the Human Mitochondrial Interactome during Neuronal Reprogramming Reveals Regulators of the Respirasome and Neurogenesis.

Moutaoufik MT, Malty R, Amin S, Zhang Q, Phanse S, Gagarinova A, Zilocchi M, Hoell L, Minic Z, Gagarinova M, Aoki H, Stockwell J, Jessulat M, Goebels F, Broderick K, Scott NE, Vlasblom J, Musso G, Prasad B, Lamantea E, Garavaglia B, Rajput A, Murayama K, Okazaki Y, Foster LJ, Bader GD, Cayabyab FS, Babu M

Mitochondrial protein (MP) assemblies undergo alterations during neurogenesis, a complex process vital in brain homeostasis and disease. Yet which MP assemblies remodel during differentiation remains unclear. Here, using mass spectrometry-based co-fractionation profiles and phosphoproteomics, we generated mitochondrial interaction maps of human pluripotent embryonal carcinoma stem cells and differentiated neuronal-like cells, which presented as two discrete cell populations by single-cell RNA ... [more]

iScience Sep. 27, 2019; 19();1114-1132 [Pubmed: 31536960]

Quantitative Score

1.797915127 [Confidence Score]

Throughput

High Throughput

Additional Notes

Protein pairs with Sigma LLS score >= 1.45 derived from Ntera2 embryonic carcinoma stem cells (ECSC)

Related interactions

Interaction	Experimental Evidence Code	Dataset	Throughput	Score	Curated By	Notes
LRPPRC NDUFS1	Proximity Label-MS Proximity Label-MS An interaction is inferred when a bait-enzyme fusion protein selectively modifies a vicinal protein with a diffusible reactive product, followed by affinity capture of the modified protein and identification by mass spectrometric methods.	Antonicka H (2020)	High	1	BioGRID	2852702

Curated By

BioGRID

Interaction Summary

NDUFS1

Gene Ontology Biological Process

Gene Ontology Molecular Function

NADH dehydrogenase (ubiquinone) activity [IMP, NAS]electron carrier activity [NAS]protein binding [IPI]

Gene Ontology Cellular Component

LRPPRC