Co-purification

An interaction is inferred from the identification of two or more protein subunits in a purified protein complex, as obtained by classical biochemical fractionation or affinity purification and one or more additional fractionation steps.

Publication

E2F-5, a new E2F family member that interacts with p130 in vivo.

Hijmans EM, Voorhoeve PM, Beijersbergen RL, van 't Veer LJ, Bernards R

E2F DNA binding sites are found in a number of genes whose expression is tightly regulated during the cell cycle. The activity of E2F transcription factors is regulated by association with specific repressor molecules that can bind and inhibit the E2F transactivation domain. For E2F-1, E2F-2, and E2F-3, the repressor is the product of the retinoblastoma gene, pRb. E2f-4 interacts ... [more]

Mol. Cell. Biol. Jun. 01, 1995; 15(6);3082-9 [Pubmed: 7760804]

Throughput

  • Low Throughput

Additional Notes

  • band shift assay

Related interactions

InteractionExperimental Evidence CodeDatasetThroughputScoreCurated ByNotes
RBL2 E2F5
Affinity Capture-MS
Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Low-BioGRID
-
RBL2 E2F5
Two-hybrid
Two-hybrid

Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation.

Low-BioGRID
-

Curated By

  • BioGRID