NCALD
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
GAPDH
Gene Ontology Biological Process
- apoptotic process [IMP]
- carbohydrate metabolic process [IDA]
- cellular response to interferon-gamma [ISO]
- gluconeogenesis [IDA]
- glycolytic process [IDA]
- microtubule cytoskeleton organization [IDA]
- multicellular organismal development [ISO]
- negative regulation of translation [ISO]
- neuron apoptotic process [IDA, IMP]
- oxidation-reduction process [ISO]
- peptidyl-cysteine S-trans-nitrosylation [IDA]
- protein stabilization [IDA]
- response to ammonium ion [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- GAIT complex [ISO, ISS]
- cytoplasm [IDA, ISO]
- cytosol [IDA, ISO]
- extracellular vesicular exosome [ISO]
- intracellular membrane-bounded organelle [ISO]
- lipid particle [ISO]
- membrane [ISO]
- microtubule cytoskeleton [IDA]
- mitochondrion [ISO]
- nuclear membrane [ISO]
- nucleus [IDA, ISO]
- plasma membrane [ISO]
- ribonucleoprotein complex [ISO]
- vesicle [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Identification of Ca2+-dependent binding partners for the neuronal calcium sensor protein neurocalcin delta: interaction with actin, clathrin and tubulin.
The neuronal calcium sensors are a family of EF-hand-containing Ca(2+)-binding proteins expressed predominantly in retinal photoreceptors and neurons. One of the family members is neurocalcin delta, the function of which is unknown. As an approach to elucidating the protein interactions made by neurocalcin delta, we have identified brain cytosolic proteins that bind to neurocalcin delta in a Ca(2+)-dependent manner. We ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| NCALD GAPDH | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - |
Curated By
- BioGRID