CLIC4
Gene Ontology Biological Process
- angiogenesis [ISO]
- branching morphogenesis of an epithelial tube [ISO]
- cellular response to calcium ion [ISO]
- chloride transmembrane transport [IDA]
- chloride transport [TAS]
- endothelial cell morphogenesis [ISO]
- fertilization [ISO]
- keratinocyte differentiation [ISO]
- multicellular organism growth [ISO]
- negative regulation of cell migration [ISO, ISS]
- retina vasculature morphogenesis in camera-type eye [ISO]
- vacuolar acidification [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [ISO]
- cell [ISO]
- cell surface [ISO]
- cell-cell junction [ISO]
- centrosome [ISO]
- cytoplasm [ISO, ISS, TAS]
- cytosol [ISO]
- endoplasmic reticulum [TAS]
- extracellular vesicular exosome [ISO]
- integral component of membrane [TAS]
- intracellular [ISO]
- microtubule cytoskeleton [ISO]
- microvillus [ISO]
- midbody [ISO]
- mitochondrion [ISO]
- nuclear matrix [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- vacuole [ISO]
- vesicle [TAS]
ACTB
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [IPI]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- NuA4 histone acetyltransferase complex [ISO]
- axon [IDA]
- blood microparticle [ISO]
- cortical cytoskeleton [ISO]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [ISO, TAS]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [ISO]
- nuclear chromatin [ISO]
- postsynaptic density [IDA]
- protein complex [IDA, ISO]
- ribonucleoprotein complex [ISO]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms.
Mammalian chloride intracellular channel (CLIC) (p64-related) proteins are widely expressed, with an unusual dual localization as both soluble and integral membrane proteins. The molecular basis for their cellular localization and ion channel activity remains unclear. To help in addressing these problems, we identified novel rat brain CLIC4 (p64H1) binding partners by affinity chromatography, mass spectrometric analysis and microsequencing. Brain CLIC4 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CLIC4 ACTB | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - |
Curated By
- BioGRID