CLIC4
Gene Ontology Biological Process
- angiogenesis [ISO]
- branching morphogenesis of an epithelial tube [ISO]
- cellular response to calcium ion [ISO]
- chloride transmembrane transport [IDA]
- chloride transport [TAS]
- endothelial cell morphogenesis [ISO]
- fertilization [ISO]
- keratinocyte differentiation [ISO]
- multicellular organism growth [ISO]
- negative regulation of cell migration [ISO, ISS]
- retina vasculature morphogenesis in camera-type eye [ISO]
- vacuolar acidification [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [ISO]
- cell [ISO]
- cell surface [ISO]
- cell-cell junction [ISO]
- centrosome [ISO]
- cytoplasm [ISO, ISS, TAS]
- cytosol [ISO]
- endoplasmic reticulum [TAS]
- extracellular vesicular exosome [ISO]
- integral component of membrane [TAS]
- intracellular [ISO]
- microtubule cytoskeleton [ISO]
- microvillus [ISO]
- midbody [ISO]
- mitochondrion [ISO]
- nuclear matrix [ISO]
- perinuclear region of cytoplasm [ISO]
- plasma membrane [ISO]
- vacuole [ISO]
- vesicle [TAS]
DNM1
Gene Ontology Biological Process
- G-protein coupled receptor internalization [IMP]
- adult locomotory behavior [ISO]
- endocytosis [ISO]
- endosome organization [ISO]
- positive regulation of synaptic vesicle recycling [IMP]
- protein tetramerization [ISO]
- receptor internalization [IMP]
- receptor-mediated endocytosis [IMP, ISO]
- sensory perception of sound [ISO]
- synaptic transmission, GABAergic [ISO]
- toxin transport [ISO]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Chloride intracellular channel protein CLIC4 (p64H1) binds directly to brain dynamin I in a complex containing actin, tubulin and 14-3-3 isoforms.
Mammalian chloride intracellular channel (CLIC) (p64-related) proteins are widely expressed, with an unusual dual localization as both soluble and integral membrane proteins. The molecular basis for their cellular localization and ion channel activity remains unclear. To help in addressing these problems, we identified novel rat brain CLIC4 (p64H1) binding partners by affinity chromatography, mass spectrometric analysis and microsequencing. Brain CLIC4 ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CLIC4 DNM1 | Affinity Capture-MS Affinity Capture-MS An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods. | Low | - | BioGRID | - | |
| CLIC4 DNM1 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - |
Curated By
- BioGRID