Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Rab7b modulates autophagic flux by interacting with Atg4B.

Kjos I, Borg Distefano M, Saetre F, Repnik U, Holland P, Jones AT, Engedal N, Simonsen A, Bakke O, Progida C

Autophagy (macroautophagy) is a highly conserved eukaryotic degradation pathway in which cytosolic components and organelles are sequestered by specialized autophagic membranes and degraded through the lysosomal system. The autophagic pathway maintains basal cellular homeostasis and helps cells adapt during stress; thus, defects in autophagy can cause detrimental effects. It is therefore crucial that autophagy is properly regulated. In this study, ... [more]

EMBO Rep. Dec. 01, 2016; 18(10);1727-1739 [Pubmed: 28835545]

Throughput

  • Low Throughput

Curated By

  • BioGRID