PACSIN3
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
TUBG1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
- apical part of cell [IDA]
- cell leading edge [IDA]
- centriole [IDA]
- centrosome [IDA, ISO]
- ciliary basal body [IDA]
- cilium [IDA]
- condensed nuclear chromosome [IDA]
- cytoplasm [IDA, ISO]
- cytoplasmic microtubule [IDA]
- gamma-tubulin complex [ISO]
- microtubule organizing center [TAS]
- nonmotile primary cilium [IDA]
- pericentriolar material [IDA]
- polar microtubule [ISO]
- recycling endosome [ISO]
- spindle microtubule [IDA]
- spindle pole [TAS]
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
PACSIN proteins bind tubulin and promote microtubule assembly.
PACSINs are intracellular adapter proteins involved in vesicle transport, membrane dynamics and actin reorganisation. In this study, we report a novel role for PACSIN proteins as components of the centrosome involved in microtubule dynamics. Glutathione S-transferase (GST)-tagged PACSIN proteins interacted with protein complexes containing alpha- and gamma-tubulin in brain homogenate. Analysis of cell lysates showed that all three endogenous PACSINs ... [more]
Throughput
- Low Throughput
Curated By
- BioGRID