WRN
Gene Ontology Biological Process
- ATP catabolic process [IDA]
- DNA duplex unwinding [IDA, IMP]
- DNA metabolic process [IDA]
- DNA replication [IMP]
- DNA synthesis involved in DNA repair [IDA]
- aging [NAS]
- base-excision repair [IDA]
- cell aging [IMP]
- cellular response to DNA damage stimulus [IDA]
- cellular response to gamma radiation [IDA]
- cellular response to starvation [IDA]
- double-strand break repair [IMP]
- multicellular organismal aging [IMP]
- nucleic acid phosphodiester bond hydrolysis [IDA]
- nucleolus to nucleoplasm transport [IDA]
- positive regulation of hydrolase activity [IDA]
- regulation of apoptotic process [IGI]
- replication fork processing [IDA, IMP]
- response to UV-C [IDA]
- response to oxidative stress [IDA]
- telomere maintenance [IMP]
Gene Ontology Molecular Function- 3'-5' DNA helicase activity [IDA]
- 3'-5' exonuclease activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA helicase activity [IDA, IMP]
- G-quadruplex DNA binding [IDA]
- Y-form DNA binding [IDA]
- bubble DNA binding [IDA]
- exonuclease activity [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- protein homodimerization activity [IDA]
- 3'-5' DNA helicase activity [IDA]
- 3'-5' exonuclease activity [IDA]
- ATP-dependent DNA helicase activity [IDA]
- ATPase activity [IDA]
- DNA binding [IDA]
- DNA helicase activity [IDA, IMP]
- G-quadruplex DNA binding [IDA]
- Y-form DNA binding [IDA]
- bubble DNA binding [IDA]
- exonuclease activity [IDA]
- four-way junction helicase activity [IDA]
- helicase activity [IDA]
- magnesium ion binding [IDA]
- manganese ion binding [IDA]
- protein binding [IPI]
- protein complex binding [IDA]
- protein homodimerization activity [IDA]
Gene Ontology Cellular Component
MDM2
Gene Ontology Biological Process
- DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest [IMP, TAS]
- Fc-epsilon receptor signaling pathway [TAS]
- cellular response to hypoxia [IEP]
- epidermal growth factor receptor signaling pathway [TAS]
- establishment of protein localization [IDA]
- fibroblast growth factor receptor signaling pathway [TAS]
- innate immune response [TAS]
- negative regulation of DNA damage response, signal transduction by p53 class mediator [IDA]
- negative regulation of cell cycle arrest [IDA]
- negative regulation of transcription from RNA polymerase II promoter [IDA]
- negative regulation of transcription, DNA-templated [IDA]
- neurotrophin TRK receptor signaling pathway [TAS]
- peptidyl-lysine modification [IMP]
- phosphatidylinositol-mediated signaling [TAS]
- positive regulation of cell proliferation [TAS]
- positive regulation of mitotic cell cycle [IMP]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- protein complex assembly [IDA]
- protein destabilization [IDA]
- protein localization to nucleus [IDA]
- protein ubiquitination [IDA]
- protein ubiquitination involved in ubiquitin-dependent protein catabolic process [IDA]
- regulation of protein catabolic process [IDA]
- response to antibiotic [IEP]
- synaptic transmission [TAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
MDM2-mediated degradation of WRN promotes cellular senescence in a p53-independent manner.
MDM2 (Murine double minute 2) acts as a key repressor for p53-mediated tumor-suppressor functions, which includes cellular senescence. We found that MDM2 can promote cellular senescence by modulating WRN stability. Werner syndrome (WS), caused by mutations of the WRN gene, is an autosomal recessive disease, which is characterized by premature aging. Loss of WRN function induces cellular senescence in human ... [more]
Throughput
- Low Throughput
Additional Notes
- assayed using GST (glutathione S-transferase) pull-down experiments
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| WRN MDM2 | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MDM2 WRN | Affinity Capture-Western Affinity Capture-Western An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins. | Low | - | BioGRID | - | |
| MDM2 WRN | Biochemical Activity Biochemical Activity An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation. | Low | - | BioGRID | 2906400 | |
| MDM2 WRN | Co-localization Co-localization Interaction inferred from two proteins that co-localize in the cell by indirect immunofluorescence only when in addition, if one gene is deleted, the other protein becomes mis-localized. Also includes co-dependent association of proteins with promoter DNA in chromatin immunoprecipitation experiments. | Low | - | BioGRID | - |
Curated By
- BioGRID