Affinity Capture-MS

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner is identified by mass spectrometric methods.

Publication

H4K20me0 recognition by BRCA1-BARD1 directs homologous recombination to sister chromatids.

Nakamura K, Saredi G, Becker JR, Foster BM, Nguyen NV, Beyer TE, Cesa LC, Faull PA, Lukauskas S, Frimurer T, Chapman JR, Bartke T, Groth A

Genotoxic DNA double-strand breaks (DSBs) can be repaired by error-free homologous recombination (HR) or mutagenic non-homologous end-joining1. HR supresses tumorigenesis1, but is restricted to the S and G2 phases of the cell cycle when a sister chromatid is present2. Breast cancer type 1 susceptibility protein (BRCA1) promotes HR by antagonizing the anti-resection factor TP53-binding protein 1(53BP1) (refs. 2-5), but it ... [more]

Nat. Cell Biol. Dec. 01, 2018; 21(3);311-318 [Pubmed: 30804502]

Throughput

  • High Throughput

Additional Notes

  • assayed using SILAC IP (stable isotope labeling by amino acids in cell culture-based quantitative immunoprecipitation)

Curated By

  • BioGRID