ACTB
Gene Ontology Biological Process
Gene Ontology Molecular Function- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- RNA polymerase II core promoter proximal region sequence-specific DNA binding [ISO]
- RNA polymerase II distal enhancer sequence-specific DNA binding [ISO]
- Tat protein binding [ISO]
- kinesin binding [ISO]
- nitric-oxide synthase binding [ISO]
- nucleosomal DNA binding [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
Gene Ontology Cellular Component
- MLL5-L complex [ISO]
- NuA4 histone acetyltransferase complex [ISO]
- axon [ISO]
- blood microparticle [ISO]
- cortical cytoskeleton [IDA]
- cytoplasmic ribonucleoprotein granule [ISO]
- cytosol [IDA]
- extracellular space [ISO]
- extracellular vesicular exosome [ISO]
- focal adhesion [ISO]
- membrane [ISO]
- myelin sheath [IDA]
- nuclear chromatin [ISO]
- postsynaptic density [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [ISO]
CPNE1
Gene Ontology Biological Process
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Reconstituted Complex
An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.
Publication
Identification of targets for calcium signaling through the copine family of proteins. Characterization of a coiled-coil copine-binding motif.
We provide evidence that copines, members of a ubiquitous family of calcium-dependent, membrane-binding proteins, may represent a universal transduction pathway for calcium signaling because we find copines are capable of interacting with a wide variety of "target" proteins including MEK1, protein phosphatase 5, and the CDC42-regulated kinase, that are themselves components of intracellular signaling pathways. The copine target proteins were ... [more]
Throughput
- Low Throughput
Related interactions
| Interaction | Experimental Evidence Code | Dataset | Throughput | Score | Curated By | Notes |
|---|---|---|---|---|---|---|
| CPNE1 ACTB | Two-hybrid Two-hybrid Bait protein expressed as a DNA binding domain (DBD) fusion and prey expressed as a transcriptional activation domain (TAD) fusion and interaction measured by reporter gene activation. | Low | - | BioGRID | - |
Curated By
- BioGRID