BAIT
STUB1
CHIP, HSPABP2, NY-CO-7, SCAR16, SDCCAG7, UBOX1, LA16c-313D11.6
STIP1 homology and U-box containing protein 1, E3 ubiquitin protein ligase
GO Process (15)
GO Function (15)
GO Component (10)
Gene Ontology Biological Process
- cellular response to misfolded protein [IDA]
- misfolded or incompletely synthesized protein catabolic process [IDA]
- negative regulation of transforming growth factor beta receptor signaling pathway [TAS]
- positive regulation of chaperone-mediated protein complex assembly [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IDA]
- positive regulation of protein ubiquitination [IDA]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IDA]
- protein K63-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein maturation [TAS]
- protein polyubiquitination [IDA, IMP]
- regulation of glucocorticoid metabolic process [IDA]
- transforming growth factor beta receptor signaling pathway [TAS]
- ubiquitin-dependent SMAD protein catabolic process [IDA]
- ubiquitin-dependent protein catabolic process [IMP]
Gene Ontology Molecular Function- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
- G-protein coupled receptor binding [IPI]
- Hsp70 protein binding [IDA]
- Hsp90 protein binding [IDA]
- SMAD binding [IDA]
- TPR domain binding [IDA]
- enzyme binding [IPI]
- kinase binding [IPI]
- misfolded protein binding [IDA]
- protein binding [IPI]
- protein binding, bridging [TAS]
- protein homodimerization activity [ISS]
- ubiquitin protein ligase activity [IDA]
- ubiquitin protein ligase binding [IPI]
- ubiquitin-protein transferase activity [IDA, IMP, TAS]
- ubiquitin-ubiquitin ligase activity [ISS]
Gene Ontology Cellular Component
Homo sapiens
PREY
TLR4
toll-like receptor 4
GO Process (80)
GO Function (5)
GO Component (8)
Gene Ontology Biological Process
- B cell proliferation involved in immune response [ISO]
- I-kappaB phosphorylation [ISO]
- activation of MAPK activity [ISO]
- activation of innate immune response [ISO]
- cellular response to lipopolysaccharide [IEP, ISO]
- cellular response to lipoteichoic acid [ISO]
- cellular response to mechanical stimulus [IEP, ISO]
- cellular response to retinoic acid [IEP]
- defense response to Gram-negative bacterium [ISO]
- detection of lipopolysaccharide [ISO, ISS]
- immunoglobulin mediated immune response [IEP]
- inflammatory response [IEP]
- innate immune response [TAS]
- innate immune response-activating signal transduction [IMP]
- interferon-gamma production [ISO]
- intestinal epithelial structure maintenance [ISO]
- leukotriene metabolic process [IDA]
- lipopolysaccharide-mediated signaling pathway [ISO]
- macrophage activation [ISO]
- microglial cell activation [IDA]
- negative regulation of ERK1 and ERK2 cascade [ISO]
- negative regulation of interferon-gamma production [ISO]
- negative regulation of interleukin-17 production [ISO]
- negative regulation of interleukin-23 production [ISO]
- negative regulation of interleukin-6 production [ISO]
- negative regulation of tumor necrosis factor production [ISO]
- nitric oxide production involved in inflammatory response [ISO]
- positive regulation of B cell proliferation [ISO]
- positive regulation of DNA binding [IMP]
- positive regulation of ERK1 and ERK2 cascade [ISO]
- positive regulation of I-kappaB kinase/NF-kappaB signaling [ISO]
- positive regulation of JNK cascade [ISO]
- positive regulation of MHC class II biosynthetic process [ISO]
- positive regulation of NF-kappaB import into nucleus [ISO]
- positive regulation of NF-kappaB transcription factor activity [IMP, ISO]
- positive regulation of apoptotic process [IMP]
- positive regulation of chemokine production [ISO]
- positive regulation of inflammatory response [IMP]
- positive regulation of interferon-alpha production [ISO]
- positive regulation of interferon-beta biosynthetic process [ISO]
- positive regulation of interferon-beta production [ISO]
- positive regulation of interferon-gamma production [ISO]
- positive regulation of interleukin-1 production [ISO]
- positive regulation of interleukin-10 production [ISO]
- positive regulation of interleukin-12 biosynthetic process [ISO]
- positive regulation of interleukin-12 production [ISO]
- positive regulation of interleukin-6 production [ISO]
- positive regulation of interleukin-8 biosynthetic process [ISO]
- positive regulation of interleukin-8 production [ISO]
- positive regulation of lymphocyte proliferation [ISO]
- positive regulation of macrophage cytokine production [ISO]
- positive regulation of nitric oxide biosynthetic process [ISO]
- positive regulation of nitric-oxide synthase biosynthetic process [ISO]
- positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway [ISO]
- positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway [ISO]
- positive regulation of peptidyl-tyrosine phosphorylation [IDA]
- positive regulation of platelet activation [ISO]
- positive regulation of sequence-specific DNA binding transcription factor activity [IMP]
- positive regulation of stress-activated MAPK cascade [ISO]
- positive regulation of transcription from RNA polymerase II promoter [ISO]
- positive regulation of tumor necrosis factor biosynthetic process [ISO]
- positive regulation of tumor necrosis factor production [ISO]
- regulation of inflammatory response [ISO]
- regulation of sensory perception of pain [IMP]
- response to activity [IEP]
- response to bacterium [ISO]
- response to ethanol [IMP]
- response to fatty acid [IDA]
- response to glucocorticoid [IEP]
- response to gold nanoparticle [IEP]
- response to hypoxia [IEP]
- response to insulin [IEP]
- response to lipopolysaccharide [IDA, IEP, ISO]
- response to morphine [IEP]
- response to oxidative stress [IMP]
- response to progesterone [IEP]
- response to retinoic acid [IEP]
- response to toxic substance [IEP]
- skeletal muscle contraction [IEP]
- toll-like receptor signaling pathway [IDA]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
Rattus norvegicus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CHIP attenuates lipopolysaccharide-induced cardiac hypertrophy and apoptosis by promoting NFATc3 proteasomal degradation.
Carboxyl-terminus of Hsc70 interacting protein (CHIP) is a chaperone-dependent E3-ubiquitin ligase with important function in protein quality control system. In the current research endeavor, we have investigated the putative role of CHIP in lipopolysaccharides (LPS)-induced cardiomyopathies. Basically, H9c2 cardiomyoblasts were transfected with CHIP for 24 hr, and thereafter, treated with LPS for 12 hr. Concomitantly, western blot analysis, actin staining, ... [more]
J. Cell. Physiol. Nov. 01, 2019; 234(11);20128-20138 [Pubmed: 30980393]
Throughput
- Low Throughput
Additional Notes
- Source of CHIP not clear
Curated By
- BioGRID