BAIT
GSK3B
7330414F15Rik, 8430431H08Rik, C86142, GSK-3, GSK-3beta, GSK3
glycogen synthase kinase 3 beta
GO Process (56)
GO Function (17)
GO Component (19)
Gene Ontology Biological Process
- ER overload response [IDA, ISO]
- Wnt signaling pathway [IGI]
- axonogenesis [IGI]
- canonical Wnt signaling pathway [IDA, ISO]
- canonical Wnt signaling pathway involved in positive regulation of apoptotic process [IMP]
- cell migration [IGI]
- cell proliferation [TAS]
- cellular response to interleukin-3 [IDA]
- cellular response to mechanical stimulus [ISO]
- circadian rhythm [IMP]
- cytoskeleton organization [TAS]
- epithelial to mesenchymal transition [ISO]
- establishment of cell polarity [ISO]
- establishment or maintenance of cell polarity [ISO]
- extrinsic apoptotic signaling pathway in absence of ligand [IDA]
- fat cell differentiation [IDA]
- glycogen metabolic process [ISO]
- hippocampus development [ISO]
- hypermethylation of CpG island [IMP]
- intracellular signal transduction [ISO]
- myoblast fusion [IDA, IGI]
- myotube differentiation [IGI]
- negative regulation of MAP kinase activity [ISO]
- negative regulation of NFAT protein import into nucleus [ISO]
- negative regulation of apoptotic process [IMP, ISO]
- negative regulation of cardiac muscle hypertrophy [IDA]
- negative regulation of dendrite morphogenesis [ISO]
- negative regulation of neuron maturation [IGI]
- negative regulation of neuron projection development [IGI, IMP]
- negative regulation of protein binding [ISO]
- negative regulation of protein complex assembly [ISO]
- organ morphogenesis [IMP]
- peptidyl-serine phosphorylation [IDA, ISO]
- phosphorylation [IMP]
- positive regulation of Rac GTPase activity [ISO]
- positive regulation of apoptotic process [ISO]
- positive regulation of axon extension [IGI]
- positive regulation of cell-matrix adhesion [ISO]
- positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway [IDA]
- positive regulation of peptidyl-serine phosphorylation [IDA]
- positive regulation of peptidyl-threonine phosphorylation [IDA]
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process [IGI]
- positive regulation of protein binding [IDA]
- positive regulation of protein complex assembly [ISO]
- positive regulation of protein export from nucleus [ISO]
- positive regulation of stem cell differentiation [IMP]
- positive regulation of transcription from RNA polymerase II promoter [IMP]
- protein export from nucleus [IDA]
- protein localization to microtubule [IGI]
- protein phosphorylation [IDA, IGI, IMP, ISO, ISS]
- re-entry into mitotic cell cycle [IDA]
- regulation of gene expression by genetic imprinting [IMP]
- regulation of microtubule-based process [IDA, ISO]
- regulation of neuron projection development [IGI]
- regulation of neuronal synaptic plasticity [ISO]
- superior temporal gyrus development [ISO]
Gene Ontology Molecular Function- ATP binding [ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [IPI, ISO]
- integrin binding [ISO]
- ionotropic glutamate receptor binding [ISO]
- kinase activity [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, IMP, ISO, ISS]
- tau protein binding [ISO]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
- ATP binding [ISO]
- NF-kappaB binding [ISO]
- RNA polymerase II transcription factor binding [ISO]
- beta-catenin binding [IPI, ISO]
- integrin binding [ISO]
- ionotropic glutamate receptor binding [ISO]
- kinase activity [ISO]
- p53 binding [ISO]
- protein binding [IPI]
- protein kinase A catalytic subunit binding [ISO]
- protein kinase activity [IDA]
- protein kinase binding [ISO]
- protein serine/threonine kinase activity [IDA, IMP, ISO, ISS]
- tau protein binding [ISO]
- tau-protein kinase activity [IDA, ISO]
- transcription factor binding [ISO]
- ubiquitin protein ligase binding [ISO]
Gene Ontology Cellular Component
- beta-catenin destruction complex [IDA, ISO]
- cell body [IDA]
- centrosome [ISO]
- cytoplasm [ISO]
- cytosol [IDA, ISO]
- dendritic shaft [IDA]
- dendritic spine [ISO]
- growth cone [IDA]
- membrane [ISO]
- membrane raft [ISO]
- membrane-bounded organelle [IDA]
- neuronal cell body [IDA]
- neuronal postsynaptic density [IDA]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [IDA]
- plasma membrane [ISO]
- protein complex [ISO]
- ribonucleoprotein complex [IDA]
Mus musculus
PREY
CDK5R1
Cdk5r, D11Bwg0379e, p25, p35, RP23-252E4.5
cyclin-dependent kinase 5, regulatory subunit 1 (p35)
GO Process (30)
GO Function (10)
GO Component (15)
Gene Ontology Biological Process
- G-protein coupled acetylcholine receptor signaling pathway [ISO]
- axon guidance [IMP]
- axonal fasciculation [IMP]
- brain development [IMP]
- cerebellum development [IGI, IMP]
- cerebral cortex radially oriented cell migration [IMP]
- ephrin receptor signaling pathway [IMP]
- hippocampus development [IGI]
- ionotropic glutamate receptor signaling pathway [ISO]
- layer formation in cerebral cortex [IGI]
- negative regulation of axon extension [IGI]
- negative regulation of transcription, DNA-templated [ISO]
- neuron cell-cell adhesion [IMP]
- neuron differentiation [ISO]
- neuron migration [IDA, IGI]
- neuron projection development [ISO]
- peptidyl-serine phosphorylation [ISO]
- peptidyl-threonine phosphorylation [ISO]
- positive regulation of cell cycle arrest [IMP]
- positive regulation of neuron apoptotic process [ISO]
- positive regulation of protein kinase activity [IGI]
- positive regulation of protein serine/threonine kinase activity [IMP, ISO]
- positive regulation of protein targeting to membrane [IDA]
- protein phosphorylation [ISO]
- regulation of actin cytoskeleton organization [IMP]
- regulation of cell cycle [ISO]
- regulation of dendritic spine morphogenesis [IMP]
- regulation of microtubule cytoskeleton organization [IMP]
- serine phosphorylation of STAT3 protein [IDA]
- superior olivary nucleus maturation [IGI]
Gene Ontology Molecular Function- cadherin binding [ISO]
- calcium ion binding [ISO]
- cyclin-dependent protein kinase 5 activator activity [IMP, ISO, TAS]
- cyclin-dependent protein serine/threonine kinase activity [ISO]
- cytoskeletal protein binding [TAS]
- ephrin receptor binding [ISO]
- kinase activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
- cadherin binding [ISO]
- calcium ion binding [ISO]
- cyclin-dependent protein kinase 5 activator activity [IMP, ISO, TAS]
- cyclin-dependent protein serine/threonine kinase activity [ISO]
- cytoskeletal protein binding [TAS]
- ephrin receptor binding [ISO]
- kinase activity [ISO]
- protein binding [IPI]
- protein kinase binding [ISO]
- protein serine/threonine kinase activator activity [ISO]
Gene Ontology Cellular Component
- axon [ISO]
- contractile fiber [ISO]
- cyclin-dependent protein kinase 5 holoenzyme complex [ISO]
- cytoplasm [IDA, ISO]
- dendrite [ISO]
- dendritic spine [ISO]
- growth cone [ISO]
- intracellular membrane-bounded organelle [ISO]
- membrane [ISO]
- neuromuscular junction [ISO]
- neuronal cell body [ISO]
- nucleoplasm [ISO]
- nucleus [IDA, ISO]
- perinuclear region of cytoplasm [ISO]
- postsynaptic density [ISO]
Mus musculus
Affinity Capture-Western
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.
Publication
CDK5 activator protein p25 preferentially binds and activates GSK3?.
Glycogen synthase kinase 3? (GSK3?) and cyclin-dependent kinase 5 (CDK5) are tau kinases and have been proposed to contribute to the pathogenesis of Alzheimer's disease. The 3D structures of these kinases are remarkably similar, which led us to hypothesize that both might be capable of binding cyclin proteins--the activating cofactors of all CDKs. CDK5 is normally activated by the cyclin-like ... [more]
Proc. Natl. Acad. Sci. U.S.A. Nov. 11, 2014; 111(45);E4887-95 [Pubmed: 25331900]
Throughput
- Low Throughput
Curated By
- BioGRID