BAIT

ELMO1

ELMO1s
engulfment and cell motility 1
GO Process (6)
GO Function (1)
GO Component (3)
Rattus norvegicus
PREY

APBB1

FE65
amyloid beta (A4) precursor protein-binding, family B, member 1 (Fe65)
Rattus norvegicus

Affinity Capture-Western

An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and the associated interaction partner identified by Western blot with a specific polyclonal antibody or second epitope tag. This category is also used if an interacting protein is visualized directly by dye stain or radioactivity. Note that this differs from any co-purification experiment involving affinity capture in that the co-purification experiment involves at least one extra purification step to get rid of potential contaminating proteins.

Publication

Neuronal adaptor FE65 stimulates Rac1-mediated neurite outgrowth by recruiting and activating ELMO1.

Li W, Tam KMV, Chan WWR, Koon AC, Ngo JCK, Chan HYE, Lau KF

Neurite outgrowth is a crucial process in developing neurons for neural network formation. Understanding the regulatory mechanisms of neurite outgrowth is essential for developing strategies to stimulate neurite regeneration after nerve injury and in neurodegenerative disorders. FE65 is a brain-enriched adaptor that stimulates Rac1-mediated neurite elongation. However, the precise mechanism by which FE65 promotes the process remains elusive. Here, we ... [more]

J. Biol. Chem. Dec. 18, 2017; 293(20);7674-7688 [Pubmed: 29615491]

Throughput

  • Low Throughput

Curated By

  • BioGRID