Reconstituted Complex

An interaction is inferred between proteins in vitro. This can include proteins in recombinant form or proteins isolated directly from cells with recombinant or purified bait. For example, GST pull-down assays where a GST-tagged protein is first isolated and then used to fish interactors from cell lysates are considered reconstituted complexes (e.g. PUBMED: 14657240, Fig. 4A or PUBMED: 14761940, Fig. 5). This can also include gel-shifts, surface plasmon resonance, isothermal titration calorimetry (ITC) and bio-layer interferometry (BLI) experiments. The bait-hit directionality may not be clear for 2 interacting proteins. In these cases the directionality is up to the discretion of the curator.

Publication

Hook proteins: association with Alzheimer pathology and regulatory role of hook3 in amyloid beta generation.

Herrmann L, Wiegmann C, Arsalan-Werner A, Hilbrich I, Jaeger C, Flach K, Suttkus A, Lachmann I, Arendt T, Holzer M

Defects in intracellular transport are implicated in the pathogenesis of Alzheimer's disease (AD). Hook proteins are a family of cytoplasmic linker proteins that participate in endosomal transport. In this study we show that Hook1 and Hook3 are expressed in neurons while Hook2 is predominantly expressed in astrocytes. Furthermore, Hook proteins are associated with pathological hallmarks in AD; Hook1 and Hook3 ... [more]

PLoS ONE Mar. 24, 2015; 10(3);e0119423 [Pubmed: 25799409]

Throughput

  • Low Throughput

Curated By

  • BioGRID