Affinity Capture-RNA
An interaction is inferred when a bait protein is affinity captured from cell extracts by either polyclonal antibody or epitope tag and associated RNA species identified by Northern blot, RT-PCR, affinity labeling, sequencing, or microarray analysis.
Publication
LUCAT1 promotes colorectal cancer tumorigenesis by targeting the ribosomal protein L40-MDM2-p53 pathway through binding with UBA52.
Colorectal cancer (CRC) is the third most commonly diagnosed cancer in both men and women in the USA. However, the underlying molecular mechanisms that drive CRC tumorigenesis are still not clear. Several studies have reported that long noncoding RNAs (lncRNAs) have important roles in tumor development. Here, we undertook a transcriptome microarray analysis in 6 pairs of CRC tissues and ... [more]
Cancer Sci. Apr. 01, 2019; 110(4);1194-1207 [Pubmed: 30690837]
Throughput
- High Throughput
Additional Notes
- assayed using ChiRP (chromatin isolation by RNA purification) to pull down the bait LUCAT1 RNA, identifying the indicated hit proteins
Curated By
- BioGRID