UBE2A
Gene Ontology Biological Process
- DNA repair [IGI]
- antigen processing and presentation of peptide antigen via MHC class I [TAS]
- histone H2A ubiquitination [IDA]
- positive regulation of cell proliferation [IDA]
- postreplication repair [NAS]
- proteasome-mediated ubiquitin-dependent protein catabolic process [IBA]
- protein K11-linked ubiquitination [IDA]
- protein K48-linked ubiquitination [IDA]
- protein autoubiquitination [IDA]
- protein polyubiquitination [TAS]
- response to UV [IGI]
- ubiquitin-dependent protein catabolic process [NAS]
Gene Ontology Molecular Function
Gene Ontology Cellular Component
EEF1D
Gene Ontology Biological Process
Gene Ontology Molecular Function
Biochemical Activity (Ubiquitination)
An interaction is inferred from the biochemical effect of one protein upon another, for example, GTP-GDP exchange activity or phosphorylation of a substrate by a kinase. The bait protein executes the activity on the substrate hit protein. A Modification value is recorded for interactions of this type with the possible values Phosphorylation, Ubiquitination, Sumoylation, Dephosphorylation, Methylation, Prenylation, Acetylation, Deubiquitination, Proteolytic Processing, Glucosylation, Nedd(Rub1)ylation, Deacetylation, No Modification, Demethylation.
Publication
RNF20/40-mediated eEF1B?L monoubiquitylation stimulates transcription of heat shock-responsive genes.
RNF20/40 E3 ubiquitin ligase-mediated histone H2B monoubiquitylation plays important roles in many cellular processes, including transcriptional regulation. However, the multiple defects observed in RNF20-depleted cells suggest additional ubiquitylation targets of RNF20/40 beyond histone H2B. Here, using biochemically defined assays employing purified factors and cell-based analyses, we demonstrate that RNF20/40, in conjunction with its cognate E2 ubiquitin-conjugating enzyme RAD6, monoubiquitylates lysine ... [more]
Throughput
- Low Throughput
Additional Notes
- in vitro ubiquitination with Ube1 as E1, Rad6A as E2, RNF20 and RNF40 as E3 and eEF1BdeltaL as substrate
- the ubiquitination can also occur in the absence of RNF20 and RNF40
Curated By
- BioGRID